All Products
AbC- mab antibody against C-terminus AviTag
more details...Purified mouse monoclonal IgG antibody which will react specifically against the AviTag™ peptide sequence when fused to the C-terminus of your desired protein-of-interest. May be used to identify C-terminus AviTag™ fusion proteins on Western blots, in ELISAs or in any other immunological procedure when used in combination with a secondary labeled anti-mouse antibody.
AVB101 - E.coli B strain for efficient in vivo biotinylation
This E.coli B strain contains the birA gene on a plasmid. IPTG induction allows co-expression of BirA and subsequent biotinylation of your own protein cloned into our AviTagTM vectors.more details...Expresso Biotin Cloning & Expression System
CLICK HERE to access Lucigen's website for ordering Expresso® Biotin Cloning & Expression System products, additional information and protocolsmore details...BirA500: BirA biotin-protein ligase standard reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 40ug of BirA enzyme and all necessary buffers for those who require smaller scale reactions or less frequent use of the BirA biotinylation reaction in their work.more details...p3A-T5-N-Avtg-SF vector
Avidity Atum AviTag™ (AAA) vector using T5 promoter (IPTG inducible) with N-terminal AviTag continaing a short, flexible linker.more details...BirA500-RT: BirA biotin-protein ligase lyophilized reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 40ug of lyophilized BirA enzyme and all necessary buffers for those who require smaller scale reactions or less frequent use of the BirA biotinylation reaction in their work. This kit and its components can be shipped and stored at room temperature.more details...CVB101- Chemically competent cells
Chemically Competent E. coli B strain for in vivo biotinylationmore details...p3A-T5-N-Avtg-RE vector
Avidity Atum AviTag™ (AAA) vector using T5 promoter (IPTG inducible) with N-terminal AviTag containing a rigid, extended linker.more details...Bulk BirA: BirA biotin-protein ligase bulk reaction kit
more details...The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 300 µg of BirA enzyme for those who require larger scale reactions or more frequent use of the BirA biotinylation reaction in their work.
p3A-T5-C-Avtg-SF vector
Avidity Atum AviTag™ (AAA) vector using T5 promoter (IPTG inducible) with C-terminal AviTag continaing a short, flexible linker.more details...Bulk BirA-RT: BirA biotin-protein ligase bulk lyophilized reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 300 µg of lyophilized BirA enzyme for those who require larger scale reactions or more frequent use of the BirA biotinylation reaction in their work. This kit and its components can be shipped and stored at room temperature.more details...p3A-T5-C-Avtg-RE vector
Avidity Atum AviTag™ (AAA) vector using T5 promoter (IPTG inducible) with C-terminal AviTag continaing a rigid, extended linker.more details...BIS-300 positive and negative control protein kit
Positive control biotinylation substrate and fully biotinylated assay standardmore details...p3A-Rha-N-Avtg-SF vector
Avidity Atum AviTag™ (AAA) vector using Rhamnose promoter (Rha inducible) with N-terminal AviTag continaing a shot, flexible linker.more details...BIO-200 biotin solution
more details...1.5mL of 500µM d-biotin solution (10 mM Bicine, pH 8.3) for BirA biotin-protein ligase reaction conditions that may require extra biotin. This is the same d-biotin solution as provided in the BirA-500 and Bulk BirA in-vitro biotinylation reaction kits. Suitable for supplimenting in vitro biotin ligase reactions. Solution is shipped at room temperature and should be stored at -20°C upon receipt until use.
Supplied as: 1 vial of d-biotin solution; 1.5mL at 500µM store at -20°C
EVB101- Electro Competent E. coli B strain for in vivo biotinylation
EVB101 are electro-competent AVB101 cells. Electroporation efficiency is >1x1010 transformants per μg of plasmid DNA.more details...p3A-Rha-N-Avtg-RE vector
Avidity Atum AviTag™ (AAA) vector using a Rhamnose promoter (Rha inducible) with N-terminal AviTag containing a rigid, extended linker.more details...p3A-Rha-C-Avtg-SF vector
Avidity Atum AviTag™ (AAA) vector using a Rhamnose promoter (Rha inducible) with C-terminal AviTag containing a short, flexible linker.more details...pBirAcm - plasmid for creation of BirA-overproducing E.coli cell line
The pBirAcm plasmid can be transformed into an E.coli strain creating a BirA overproducing cell line.more details...AVB100 - Glycerol Stock
more details...AVB100 is an E. coli K12 strain with the biotin ligase gene stably integrated into the chromosome.
BIO-500 biotin solution
10 ml Biotin solution at higher concentration (5 mM) in 10 mM Bicine (pH 8.3). Suitable for supplimenting induction growth media and large scale biotin ligase reactions. Solution is filter sterilized. Solution is shipped at room temperature. Solution should be stored at -20oC upon receipt until use.more details...p3A-Rha-C-Avtg-RE vector
Avidity Atum AviTag™ (AAA) vector using the Rhamnose promoter (Rha inducible) with C-terminal AviTag containing a rigid, extended linker.more details...PACs
A set of 3 plasmids (pAC4, pAC5, and pAC6) for cloning and expression of C-terminal AviTagged proteins in each reading frame. 10 ug of each plasmid. Maps and more information.more details...- Electra Cloning KitThe Electra Cloning Kit is necessary for use with the Avidity Atum AviTag™ (p3) vectors.more details...
pAN4
The pAN series of vectors are used to express N-terminal AviTag-protein fusions. Cloning stratagy must place the gene of interest in frame with the AviTag sequence. Maps and further information.more details...BIP-300 - positive control plasmid for induction
more details...This plasmid allows you to compare your pAN or pAC AviTagTM fusion gene's protein expression against a confirmed AviTagTM fusion product under the same growth and induction conditions.
pAN5
he pAN series of vectors are used to express N-terminal AviTag-protein fusions. Cloning stratagy must place the gene of interest in frame with the AviTag sequence. Maps and further information.more details...pAN6
The pAN series of vectors are used to express N-terminal AviTag-protein fusions. Cloning stratagy must place the gene of interest in frame with the AviTag sequence. Maps and further information.more details...PANs
A set of 3 plasmids (pAN4, pAN5, and pAN6) for cloning and expression of N-terminal AviTagged proteins in each reading frame. 10 ug of each plasmid. Maps and more information.more details...Biotinylated Twinkle® CFP - 100 ug
Biotinylated fluorescent protein that is C-terminal fused to an Avitag™: 100 ug as a 1.0 mg/mL stock (100 uL total). Please see the specification sheet.more details...- Biotinylated Holly® CFP - 100 ugBiotinylated fluorescent protein that is C-terminal fused to an Avitag™: 100 ug as a 1.0 mg/mL stock (100 uL total). Please see the specification sheet.more details...
- Biotinylated Dasher® CFP - 100 ugBiotinylated fluorescent protein that is C-terminal fused to an Avitag™: 100 ug as a 1.0 mg/mL stock (100 uL total). Please see the specification sheet.more details...
- Fluorescent Streptavidin Twinkle® Fusion Protein - 100 ugFluorescent protein that are C-teminal fused to the Avitag™ and conjugated to Streptavidin: 100 ug as a 0.5 mg/mL stock (200 uL total). Please see the specification sheet.more details...
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