Avidity teamed with Atum (formerly DNA 2.0) to offer our customers an easy solution to AviTagging their E. coli expressed proteins. The vectors employ the Atum Electra™ system for easy cloning from a PCR product.
To minimize the steric conflicts that can affect efficient biotinylation of the AviTag, Avidity's vectors offer the choice of a short, flexible linker or an extended, semi-rigid linker between the AviTag and the target protein in either N- or C-terminal presentation of the AviTag. Our vectors use either the T5 promoter or rhamnose promoter.
This Avidity Atum AviTag™ (AAA) vector uses the T5 promoter (IPTG inducible) with a C-terminal AviTag containing a rigid, extended linker. Please note that vector is a linearized plasmid although the map of the vector is shown as circular for presentation purposes (see vector map under Other Tools and Resources tab).
Cloning Vectors and Electra Cloning
The vectors is supplied as 200ng linearized DNA (10 microliters, 20ng/ microliter) or enough for 10 cloning reactions (see Cloning Protocol).
Every vector kit is supplied with a positive control that when transformed into an E. coli strain produces a colored (magenta) colony.