Development of a multifunctional tool for drug screening against plasmodial protein-protein interactions via surface plasmon resonance
Author: Boucher LE, Bosch J
Very interesting paper about the use of the Avitag in combination with plasmon resonance to characterize protein-protein interaction. J Mol Recognit. 2013 Oct;26(10):496-500
Single reporter for targeted multimodal in vivo imaging.
Author: Niers JM, Chen JW, Lewandrowski G, Kerami M, Garanger E, Wojtkiewicz G, Waterman P, Keliher E, Weissleder R, Tannous BA.
In vivo biotinylation of Gaussia Luciferase in Glioma cells. J Am Chem Soc. 2012 Mar 21;134(11):5149-56
Gaussia luciferase variant for high-throughput functional screening applications.
Author: Maguire CA, Deliolanis NC, Pike L, Niers JM, Tjon-Kon-Fat LA, Sena-Esteves M, Tannous BA.
Anal Chem. 2009 Aug 15;81(16):7102-6. PubMed PMID: 19601604
Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence.
Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence. Author: Welsh JP, Patel KG, Manthiram K, Swartz JR.
Biochem Biophys Res Commun. 2009 Nov 27;389(4):563-8. PMID: 19825431.
Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization.
Author: Verhaegent M, Christopoulos TK.
Anal Chem. 2002 Sep 1;74(17):4378-85. PubMed PMID: 12236345.
Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization
Author: Verhaegent M, Christopoulos TK
Anal Chem. 2002 Sep 1;74(17):4378-85
A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation
Author: Beckett D, Kovaleva E, Schatz PJ
This paper shows the kinetics of another precursor of the AviTag with the following peptide sequence: GLNDIFEAQKIEWH. The addition of Glutamic Acid (E) at the end improved the kinetics another 4-fold leading to the final AviTag with following sequence: GLNDIFEAQKIEWHE. Protein Sci. 1999 Apr;8(4):921-9
Development of a multiplex bead-based assay to monitor dengue virus seroconversion Author: Ng K, Connolly JE
Author: Ng K, Connolly JE
A very recent application where the Avitag is used for a biotin / avidin-bead based multiplex assay. Methods Mol Biol. 2014;1138:53-74
Purification of a rat neurotensin receptor expressed in Escherichia coli
Author: Tucker J, Grisshammer R
This paper compares different tags to the Bio-Tag a precursor of the improved AviTag. Biochem J. 1996 Aug 1;317 ( Pt 3):891-9.
Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation
Author: Schatz PJ
First description of a 13 aa peptide that can be biotinylated as well as its natural 75 aa substrate. Biotechnology (N Y). 1993 Oct;11(10):1138-43
Cardiotrophin-like cytokine labelling using Bir A biotin ligase: a sensitive tool to study receptor expression by immune and non-immune cells
Author: Cognet I, Guilhot F, Gabriac M, Chevalier S, Chouikh Y, Herman-Bert A, Guay-Giroux A, Corneau S, Magistrelli G, Elson GC, Gascan H, Gauchat JF
This paper describes the in vitro biotinylation of Avitagged Cytokines with BirA biotin ligase. J Immunol Methods. 2005 Jun;301(1-2):53-65.
Construction of an agglutination tool: recombinant Fab fragments biotinylated in vitro
Author: Czerwinski M, Krop-Watorek A, Wasniowska K, Smolarek D, Spitalnik SL
This paper describes the in vitro biotinylation of Fab fragments using Avidity's BirA enzyme. N Biotechnol. 2009 Nov 30;26(5):215-21.
Recycling of the Ca2+-activated K+ channel, KCa2.3, is dependent upon RME-1, Rab35/EPI64C, and an N-terminal domain
Author: Gao Y, Balut CM, Bailey MA, Patino-Lopez G, Shaw S, Devor DC
Very interesting paper about biotinylation of surface (membrane) proteins using Avidity's BirA enzyme. J Biol Chem. 2010 Jun 4;285(23):17938-53
A novel method for producing mono-biotinylated, biologically active neurotrophic factors: an essential reagent for single molecule study of axon
Author: Sung K, Maloney MT, Yang J, Wu C
In vivo monobiotinylation on Avitagged proteins to study axonal transport. J Neurosci Methods. 2011 Sep
A novel multi-affinity tag system to produce high levels of soluble and biotinylated proteins in Escherichia coli
Author: Ashraf SS, Benson RE, Payne ES, Halbleib CM, Gron H
Use of Avitag for in vivo biotinylation of recombinant expressed proteins. Protein Expr Purif. 2004 Feb;33(2):238-45.
Regulatable in vivo biotinylation expression system in mouse embryonic stem cells
Author: Wang Q, Wagner RT, Cooney AJ
The use of the Avitag in embryonic stem cells with a BirA knock-in mouse for in vivo biotinylation. PLoS One. 2013 May 7;8(5):e63532.