Application Examples for Using the AviTag

  • Development of a multifunctional tool for drug screening against plasmodial protein-protein interactions via surface plasmon resonance

    Author: Boucher LE, Bosch J

    Very interesting paper about the use of the Avitag in combination with plasmon resonance to characterize protein-protein interaction. J Mol Recognit. 2013 Oct;26(10):496-500

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Applications of GLuc-Avitag

  • Single reporter for targeted multimodal in vivo imaging.

    Author: Niers JM, Chen JW, Lewandrowski G, Kerami M, Garanger E, Wojtkiewicz G, Waterman P, Keliher E, Weissleder R, Tannous BA.

    In vivo biotinylation of Gaussia Luciferase in Glioma cells. J Am Chem Soc. 2012 Mar 21;134(11):5149-56

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Gaussia Luciferase

  • Gaussia luciferase variant for high-throughput functional screening applications.

    Author: Maguire CA, Deliolanis NC, Pike L, Niers JM, Tjon-Kon-Fat LA, Sena-Esteves M, Tannous BA.

    Anal Chem. 2009 Aug 15;81(16):7102-6. PubMed PMID: 19601604

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  • Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence.

    Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence. Author: Welsh JP, Patel KG, Manthiram K, Swartz JR.

    Biochem Biophys Res Commun. 2009 Nov 27;389(4):563-8. PMID: 19825431.

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  • Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization.

    Author: Verhaegent M, Christopoulos TK.

    Anal Chem. 2002 Sep 1;74(17):4378-85. PubMed PMID: 12236345.

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  • Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization

    Author: Verhaegent M, Christopoulos TK

    Anal Chem. 2002 Sep 1;74(17):4378-85

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History of the AviTag

  • A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation

    Author: Beckett D, Kovaleva E, Schatz PJ

    This paper shows the kinetics of another precursor of the AviTag with the following peptide sequence: GLNDIFEAQKIEWH. The addition of Glutamic Acid (E) at the end improved the kinetics another 4-fold leading to the final AviTag with following sequence: GLNDIFEAQKIEWHE. Protein Sci. 1999 Apr;8(4):921-9

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  • Development of a multiplex bead-based assay to monitor dengue virus seroconversion Author: Ng K, Connolly JE

    Author: Ng K, Connolly JE

    A very recent application where the Avitag is used for a biotin / avidin-bead based multiplex assay. Methods Mol Biol. 2014;1138:53-74

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  • Purification of a rat neurotensin receptor expressed in Escherichia coli

    Author: Tucker J, Grisshammer R

    This paper compares different tags to the Bio-Tag a precursor of the improved AviTag. Biochem J. 1996 Aug 1;317 ( Pt 3):891-9.

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  • Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation

    Author: Schatz PJ

    First description of a 13 aa peptide that can be biotinylated as well as its natural 75 aa substrate. Biotechnology (N Y). 1993 Oct;11(10):1138-43

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in-vitro Biotinylation

  • Cardiotrophin-like cytokine labelling using Bir A biotin ligase: a sensitive tool to study receptor expression by immune and non-immune cells

    Author: Cognet I, Guilhot F, Gabriac M, Chevalier S, Chouikh Y, Herman-Bert A, Guay-Giroux A, Corneau S, Magistrelli G, Elson GC, Gascan H, Gauchat JF

    This paper describes the in vitro biotinylation of Avitagged Cytokines with BirA biotin ligase. J Immunol Methods. 2005 Jun;301(1-2):53-65.

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  • Construction of an agglutination tool: recombinant Fab fragments biotinylated in vitro

    Author: Czerwinski M, Krop-Watorek A, Wasniowska K, Smolarek D, Spitalnik SL

    This paper describes the in vitro biotinylation of Fab fragments using Avidity's BirA enzyme. N Biotechnol. 2009 Nov 30;26(5):215-21.

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  • Recycling of the Ca2+-activated K+ channel, KCa2.3, is dependent upon RME-1, Rab35/EPI64C, and an N-terminal domain

    Author: Gao Y, Balut CM, Bailey MA, Patino-Lopez G, Shaw S, Devor DC

    Very interesting paper about biotinylation of surface (membrane) proteins using Avidity's BirA enzyme. J Biol Chem. 2010 Jun 4;285(23):17938-53

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  • BirA500: BirA biotin-protein ligase standard reaction kit

    New BirA-RT vs. Current BirA Comparison

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  • BirA500-RT: BirA biotin-protein ligase lyophilized reaction kit

    New BirA-RT vs. Current BirA Comparison

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in-vivo Biotinylation

  • A novel method for producing mono-biotinylated, biologically active neurotrophic factors: an essential reagent for single molecule study of axon

    Author: Sung K, Maloney MT, Yang J, Wu C

    In vivo monobiotinylation on Avitagged proteins to study axonal transport. J Neurosci Methods. 2011 Sep

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  • A novel multi-affinity tag system to produce high levels of soluble and biotinylated proteins in Escherichia coli

    Author: Ashraf SS, Benson RE, Payne ES, Halbleib CM, Gron H

    Use of Avitag for in vivo biotinylation of recombinant expressed proteins. Protein Expr Purif. 2004 Feb;33(2):238-45.

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  • Regulatable in vivo biotinylation expression system in mouse embryonic stem cells

    Author: Wang Q, Wagner RT, Cooney AJ

    The use of the Avitag in embryonic stem cells with a BirA knock-in mouse for in vivo biotinylation. PLoS One. 2013 May 7;8(5):e63532.

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  • Bulk BirA: BirA biotin-protein ligase bulk reaction kit

    New BirA-RT vs. Current BirA Comparison

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