• Application Examples for Using the AviTag
• Applications of GLuc-Avitag
• Gaussia Luciferase
• History of the AviTag
• in-vitro Biotinylation
• in-vivo Biotinylation

Application Examples for Using the AviTag

• Development of a multifunctional tool for drug screening against plasmodial protein-protein interactions via surface plasmon resonance
  
  Author: Boucher LE, Bosch J
  
  Very interesting paper about the use of the Avitag in combination with plasmon resonance to characterize protein-protein interaction. J Mol Recognit. 2013 Oct;26(10):496-500

     View Article

Applications of GLuc-Avitag

• Single reporter for targeted multimodal in vivo imaging.
  
  Author: Niers JM, Chen JW, Lewandrowski G, Kerami M, Garanger E, Wojtkiewicz G, Waterman P, Keliher E, Weissleder R, Tannous BA.
  
  In vivo biotinylation of Gaussia Luciferase in Glioma cells. J Am Chem Soc. 2012 Mar 21;134(11):5149-56

     View PDF

Gaussia Luciferase

• Gaussia luciferase variant for high-throughput functional screening applications.
  
  Author: Maguire CA, Deliolanis NC, Pike L, Niers JM, Tjon-Kon-Fat LA, Sena-Esteves M, Tannous BA.
  
  Anal Chem. 2009 Aug 15;81(16):7102-6. PubMed PMID: 19601604

     View Article

• Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence.
  
  Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence. Author: Welsh JP, Patel KG, Manthiram K, Swartz JR.
  
  Biochem Biophys Res Commun. 2009 Nov 27;389(4):563-8. PMID: 19825431.

     View Article

• Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization.
  
  Author: Verhaegent M, Christopoulos TK.
  
  Anal Chem. 2002 Sep 1;74(17):4378-85. PubMed PMID: 12236345.

     View Article

• Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization
  
  Author: Verhaegent M, Christopoulos TK
  
  Anal Chem. 2002 Sep 1;74(17):4378-85

     View Article

History of the AviTag

• A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation
  
  Author: Beckett D, Kovaleva E, Schatz PJ
  
  This paper shows the kinetics of another precursor of the AviTag with the following peptide sequence: GLNDIFEAQKIEWH. The addition of Glutamic Acid (E) at the end improved the kinetics another 4-fold leading to the final AviTag with following sequence: GLNDIFEAQKIEWHE. Protein Sci. 1999 Apr;8(4):921-9

     View PDF

• Development of a multiplex bead-based assay to monitor dengue virus seroconversion Author: Ng K, Connolly JE
  
  Author: Ng K, Connolly JE
  
  A very recent application where the Avitag is used for a biotin / avidin-bead based multiplex assay. Methods Mol Biol. 2014;1138:53-74

     View Article

• Purification of a rat neurotensin receptor expressed in Escherichia coli
  
  Author: Tucker J, Grisshammer R
  
  This paper compares different tags to the Bio-Tag a precursor of the improved AviTag. Biochem J. 1996 Aug 1;317 ( Pt 3):891-9.

     View PDF

• Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation
  
  Author: Schatz PJ
  
  First description of a 13 aa peptide that can be biotinylated as well as its natural 75 aa substrate. Biotechnology (N Y). 1993 Oct;11(10):1138-43

     View Article

in-vitro Biotinylation

• Cardiotrophin-like cytokine labelling using Bir A biotin ligase: a sensitive tool to study receptor expression by immune and non-immune cells
  
  Author: Cognet I, Guilhot F, Gabriac M, Chevalier S, Chouikh Y, Herman-Bert A, Guay-Giroux A, Corneau S, Magistrelli G, Elson GC, Gascan H, Gauchat JF
  
  This paper describes the in vitro biotinylation of Avitagged Cytokines with BirA biotin ligase. J Immunol Methods. 2005 Jun;301(1-2):53-65.

     View Article

• Construction of an agglutination tool: recombinant Fab fragments biotinylated in vitro
  
  Author: Czerwinski M, Krop-Watorek A, Wasniowska K, Smolarek D, Spitalnik SL
  
  This paper describes the in vitro biotinylation of Fab fragments using Avidity's BirA enzyme. N Biotechnol. 2009 Nov 30;26(5):215-21.

     View Article

• Recycling of the Ca2+-activated K+ channel, KCa2.3, is dependent upon RME-1, Rab35/EPI64C, and an N-terminal domain
  
  Author: Gao Y, Balut CM, Bailey MA, Patino-Lopez G, Shaw S, Devor DC
  
  Very interesting paper about biotinylation of surface (membrane) proteins using Avidity's BirA enzyme. J Biol Chem. 2010 Jun 4;285(23):17938-53

     View Article

• BirA500: BirA biotin-protein ligase standard reaction kit

  New BirA-RT vs. Current BirA Comparison
  
  

     View PDF

• BirA500-RT: BirA biotin-protein ligase lyophilized reaction kit

  New BirA-RT vs. Current BirA Comparison
  
  

     View PDF

in-vivo Biotinylation

• A novel method for producing mono-biotinylated, biologically active neurotrophic factors: an essential reagent for single molecule study of axon
  
  Author: Sung K, Maloney MT, Yang J, Wu C
  
  In vivo monobiotinylation on Avitagged proteins to study axonal transport. J Neurosci Methods. 2011 Sep

     View PDF

• A novel multi-affinity tag system to produce high levels of soluble and biotinylated proteins in Escherichia coli
  
  Author: Ashraf SS, Benson RE, Payne ES, Halbleib CM, Gron H
  
  Use of Avitag for in vivo biotinylation of recombinant expressed proteins. Protein Expr Purif. 2004 Feb;33(2):238-45.

     View Article

• Regulatable in vivo biotinylation expression system in mouse embryonic stem cells
  
  Author: Wang Q, Wagner RT, Cooney AJ
  
  The use of the Avitag in embryonic stem cells with a BirA knock-in mouse for in vivo biotinylation. PLoS One. 2013 May 7;8(5):e63532.

     View PDF

• Bulk BirA: BirA biotin-protein ligase bulk reaction kit

  New BirA-RT vs. Current BirA Comparison
  
  

     View PDF