Biotinylated Recombinant Proteins

Gaussia princeps Luciferase
Biotinylated Avitag™ fusion (C’terminus)

Gaussia princeps luciferase is a bioluminescent protein. It is ATP-independent, requiring only coelenterazine and O2 as substrates. This purified protein is fused with Avitag™ peptide and is fully biotinylated(MW 21,616 daltons). Gaussia princeps luciferase provides all the research possibilities of the biotin interaction with Streptavidin AND the applications of bioluminescence.

Gaussia luciferase from Gaussia princeps (a marine copepod) was genetically engineered to be produced in E.coli and to contain the Avitag peptide sequence. Avitag peptide (GLNDIFEAQKIEWHE) can be efficiently biotinylated by the E.coli enzyme, biotin ligase. The peptide can be recognized by AbC antibody.
Learn more or see individual products for specification sheet
Support product- Coelenterazine
Renilla reniformis Green Fluorescent Protein
Biotinylated Avitag™ fusion (C’terminus)
The Green Fluorescent Protein of Renilla reniformis has a diverse range of uses in cell biology, drug discovery, biomedical research, high throughput screening (HTS) and fluorescence activated cell sorting (FACS). Fluorescent proteins expressed in living cells (e.g. fungi, bacteria, animals and plants) can be used to study molecular processes such as dynamics of nuclear division, protein trafficking, etc. in real time GFPs can also be used for the dynamic monitoring of protein interactions using FRET or BRET technologies.

Renilla reniformis Green Fluorescent Protein was genetically engineered to be produced in E.coli and to contain the Avitag peptide sequence (MW=29,525 daltons). Avitag peptide (GLNDIFEAQKIEWHE) can be efficiently biotinylated by the E.coli enzyme, biotin ligase. The peptide can be recognized by
Abc antibody. The supplied protein is fully biotinylated at the biotinylation site within the Avitag sequence.

Renilla reniformis Green Fluorescent Protein provides all the research possibilities of the biotin interaction with Streptavidin AND the applications of fluorescence.

Learn more or read Specification sheet

Streptavidin-Green Fluorescent Protein(Renilla reniformis)

Very useful detection molecule for biotin or biotinylated proteins through the Steptavidin molecule. The strong fluorescence of the Renilla reniformis GFP allows sensitive detection. Potential uses include, but are not limited to:
ELISA
Immunohisto/cytochemistry
In situ hybridization
Specification sheet

Streptavidin-Luciferase(Gaussia princeps)

Very unique reagent that has a number of potential uses for detecting biotin or biotinylated proteins. The method of visualization is via the light generated by the luciferase.

Biotinylated luciferase is mixed with streptavidin (1:1 moles) to create labeled streptavidin with available biotin binding sites.

Specification sheet

Bis-300-Positive Control Substrate
Determination of extent of biotinylation
BIS-300 kit contains fully biotinylated and fully unbiotinylated Maltose Binding Protein with Avitag (MBP-AviTag) at the C-terminus. They are used to generate standard curves in a modified ELISA protocol. The extent of biotinylation of the test protein is measured by comparison with known quantities of fully biotinylated MBP-AviTag protein (MW 44,846 daltons).

The standard protein and unknown proteins are adsorbed to the wells of a 96-well plate at known protein quantities. The biotin associated with the AviTag is detected by its interaction with streptavidin-conjugated alkaline phophatase. Extraneous biotin is removed when the samples are washed after adsorption, eliminating exhaustive dialysis steps. As little as 1ng of biotinylated protein can be detected (Contrary to HABA based assays) This assay is dependent on the ability of the test proteins to bind to the plastic of the 96-well plate. The fully biotinylated and fully unbiotinylated Maltose Binding Protein with Avitag at the C-terminus kit can also be used as control samples for western blots or other applications.

Determine the extent of biotinylation with BIS-300 (BRTA assay)(pdf)
BIS-300 specification sheet (pdf)