EVB101 - EVB101- Electro Competent E. coli B strain for in vivo biotinylation,
$195.00
AVB101 cells made competent for uptake of plasmid DNA by electroporation. Efficiency of transformation is >1010 transformants per µg of supercoiled plasmid DNA.
Quantity: 10 vials, 50 µl per vial Store at -80ºC.
Product Information
Strain EVB101, an electro-competent E. coli B strain (hsdR, lon11, sulA1), contains pBirAcm , an IPTG inducible plasmid containing the BirA gene engineered into pACYC184. It is compatible with most cloning vectors and is maintained with chloramphenicol (10 μg/ml). This strain is recommended for protein expression because of its robust growth and the absence of the OmpT and Lon proteases.At the time of induction, Biotin should be added at a concentration of 50 µM. The pAN and pAC vectors containing the AviTagTM require ampicillin (100 µg/ml).
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