EVB101 - EVB101- Electro Competent E. coli B strain for in vivo biotinylation,
AVB101 cells made competent for uptake of plasmid DNA by electroporation. Efficiency of transformation is >1010 transformants per µg of supercoiled plasmid DNA.
Quantity: 10 vials, 50 µl per vial Store at -80ºC.
Strain EVB101, an electro-competent E. coli B strain (hsdR, lon11, sulA1), contains pBirAcm , an IPTG inducible plasmid containing the BirA gene engineered into pACYC184. It is compatible with most cloning vectors and is maintained with chloramphenicol (10 μg/ml). This strain is recommended for protein expression because of its robust growth and the absence of the OmpT and Lon proteases.
At the time of induction, Biotin should be added at a concentration of 50 µM. The pAN and pAC vectors containing the AviTagTM require ampicillin (100 µg/ml).
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