The pBirAcm plasmid can be transformed into an E.coli strain to create a BirA-overproducing cell line. The pBirAcm plasmid is derived from the pACYC184 low-copy-number plasmid (without the Tc resistance) and is maintained via the addition of chloramphenicol (10 - 30 µg/mL). BirA overproduction is controlled by a Tac promoter and is IPTG inducible. The plasmid is supplied in water at a concentration of 1 µg/mL for use in either chemical transformation or electroporation.