BirA Enzyme and buffers for in vitro biotinylation

BirA biotin-protein ligase kits for rapid and efficient biotinylation of purified proteins or peptides that have a biotin-acceptor peptide sequence such as our patented AviTag™.

  • BirA-500: BirA biotin-protein ligase standard reaction kit
    The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 40ug of BirA enzyme and all necessary buffers for those who require smaller scale reactions or less frequent use of the BirA biotinylation reaction in their work.
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  • Bulk BirA: BirA biotin-protein ligase bulk reaction kit

    The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 300 µg of BirA enzyme for those who require larger scale reactions or more frequent use of the BirA biotinylation reaction in their work.

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  • BIS-300 positive and negative control protein kit
    Positive control biotinylation substrate and fully biotinylated assay standard
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  • BIO-200 biotin solution

    1.5mL of 500µM d-biotin solution (10 mM Bicine, pH 8.3) for BirA biotin-protein ligase reaction conditions that may require extra biotin. This is the same d-biotin solution as provided in the BirA-500 and Bulk BirA in-vitro biotinylation reaction kits. Suitable for supplimenting in vitro biotin ligase reactions.

    Supplied as: 1 vial of d-biotin solution; 1.5mL at 500µM store long term at -20°C

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  • BIO-500 biotin solution
    10 ml Biotin solution at higher concentration (5 mM) in 10 mM Bicine (pH 8.3). Suitable for supplimenting induction growth media and large scale biotin ligase reactions. Solution is filter sterilized. Store at 4oC.
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