BirA Enzyme and buffers for in vitro biotinylation
BirA biotin-protein ligase kits for rapid and efficient biotinylation of purified proteins or peptides that have a biotin-acceptor peptide sequence such as our patented AviTag™.BirA500: BirA biotin-protein ligase standard reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 40ug of BirA enzyme and all necessary buffers for those who require smaller scale reactions or less frequent use of the BirA biotinylation reaction in their work.more details...BirA500-RT: BirA biotin-protein ligase lyophilized reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 40ug of lyophilized BirA enzyme and all necessary buffers for those who require smaller scale reactions or less frequent use of the BirA biotinylation reaction in their work. This kit and its components can be shipped and stored at room temperature.more details...Bulk BirA: BirA biotin-protein ligase bulk reaction kit
more details...The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 300 µg of BirA enzyme for those who require larger scale reactions or more frequent use of the BirA biotinylation reaction in their work.
Bulk BirA-RT: BirA biotin-protein ligase bulk lyophilized reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 300 µg of lyophilized BirA enzyme for those who require larger scale reactions or more frequent use of the BirA biotinylation reaction in their work. This kit and its components can be shipped and stored at room temperature.more details...BIS-300 positive and negative control protein kit
Positive control biotinylation substrate and fully biotinylated assay standardmore details...BIO-200 biotin solution
more details...1.5mL of 500µM d-biotin solution (10 mM Bicine, pH 8.3) for BirA biotin-protein ligase reaction conditions that may require extra biotin. This is the same d-biotin solution as provided in the BirA-500 and Bulk BirA in-vitro biotinylation reaction kits. Suitable for supplimenting in vitro biotin ligase reactions. Solution is shipped at room temperature and should be stored at -20°C upon receipt until use.
Supplied as: 1 vial of d-biotin solution; 1.5mL at 500µM store at -20°C
BIO-500 biotin solution
10 ml Biotin solution at higher concentration (5 mM) in 10 mM Bicine (pH 8.3). Suitable for supplimenting induction growth media and large scale biotin ligase reactions. Solution is filter sterilized. Solution is shipped at room temperature. Solution should be stored at -20oC upon receipt until use.more details...
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