CVB-T7 POL - CVB-T7 POL Chemically competent cells,  $195.00

CVB-T7 POL are chemically competent cells from E. coli BL21DE3 strain. The BL21DE3-T7 is the familiar T7 expression strain that carries the T7 polymerase integrated into the lamda attB plus the addition of the pBirAcm plasmid for over-production of the BirA enzyme. The pBirAcm colE1 compatible (compatible with most expression vectors) plasmid uses the IPTG inducible; Tac promoter and is co-induced with the T7 expression.

Note: Recombinant proteins made as insoluble inclusion bodies in the BL21DE3 host strain are not biotinylated efficiently even with the BirA over-production provided by the pBirAcm vector.

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We recommend maintaining the pBirAcm with chloramphenicol (10 ug/ml) until the overnight is diluted to get the cell culture growing exponentially prior to induction.

Cells are supplied as 10 vials of 100ul liquid culture in 30% glycerol.  Store at -80°C.

Note: Recombinant proteins made as insoluble inclusion bodies in the BL21DE3 host strain are not biotinylated efficiently even with the BirA over-production provided by the pBirAcm vector.

 

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