AviTag has a myriad of medical and industrial applications and its use is growing rapidly in areas including:
• Proximity assays
• Drug Screenings
The simplicity of the AviTag system allows any protein to be uniformly tethered to almost any surface — micro-titer plates, chromatography media, biosensor chips and nitrocellulose/PVDF membranes. AviTag has already demonstrated itself as a versatile tool for:
• Protein purification
• Cell sorting
• Sensitive protein detection
Enzymatic biotinylation of proteins with the AviTag is superior to chemical methods for coupling biotin to proteins. Unlike the AviTag technology, chemical coupling randomly biotinylates the protein surface which can inactivate proteins by attaching biotin to the protein catalytic or binding domains. Also, because the chemically-biotinylated protein can attach to the streptavidin surface at multiple contact points, or in different orientations, proteins can be inactivated from sterically-unfavorable surface-bound orientation. Chemical biotinylation can suffer from batch-to-batch reproducibility, and unlike the AviTag biotinylation that is tag-specific, chemical biotinylation cannot be used with heterogeneous populations of proteins.
Using Avidity-developed vectors, AviTag can be located on either the N- or C-terminus of a fusion protein, or at internal protein locations, if the peptide domain forms a surface-exposed loop accessible to the BirA enzyme. AviTag also serves as a spacer to facilitate interaction of a protein with a binding partner.
Click here to access publications regarding AviTag.
|Sensitive protein detection systems