FAQ's

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AbCA

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AbCA
  • What methods are available to elute the AviTag’d protein off the AbCA?

    Elution methods include low pH elution, polyol elution with propanediol or ethylene glycol with a non-chaotropic salt, or we have even had a customer elute their AviTag’d protein off the AbCA by competing it off with synthesized AviTag™ peptide in solution.

  • Does the AbCA resin come pre-packed in a column?

    No. The resin comes as a 4mL, 50% slurry that you pour and pack/equilibrate yourself. As a convenience we include a disposable column with a frit, a stop and a lid.

  • Does the AbC recognize AviTag™ if it is fused in tandem with a His-Tag?

    Yes, and the order does not matter. It can be AviTag™-His-Tag or His-Tag-AviTag™.

  • Does the AbC recognize both N-terminal and C-terminal AviTag™ protein fusions?

    The AbC recognizes only the C-terminal AviTag™ fusion presentation.

  • Is the AbC monoclonal or polyclonal? What species was it produces in? What form of IgG is it?

    The AbC is mouse monoclonal IgG2B.

  • Does the AbC recognize the biotinylated AviTag™ as well as the unbiotinylated form?

    Yes.

GaussiaLuciferaseprotein

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Gaussia Luciferase protein

AVB100

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AVB 100

AVB99

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AVB 99

BIP-300

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BIP-300

BirA

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BirA

EVB100

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EVB 100

CVB101

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CVB 101

BIS-300

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BIS-300

BIO-500

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BIO-500

BIO-200

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BIO-200

AVB101

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AVB 101

BiotinylationofProteins

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Biotinylation of Proteins

Other

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Other