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EVB100 electro-competent cells
SKU:
EVB100
$195.00

AVB100 cells made competent for uptake of plasmid DNA by electroporation. Efficiency of transformation is >1010 transformants per µg of supercoiled plasmid DNA.

Quantity: 10 vials, 50µl per vial

Please note: Strain AVB100 or derivatives when purchased conveys a limited use license to the end user. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Avidity, L.L.C. Please review this license on the specification sheet below before purchasing this product.

EVB100, an electro-competent E. coli K12 strain [MC1061 araD139 delta (ara-leu)7696 delta(lac)l74 galU galK hsdR2(rK-mK+) mcrB1 rpsL(StrR)], has a birA gene stably integrated into the chromosome. Overexpression of the BirA protein is accomplished by induction with L-arabinose (0.2-0.4%). The stably integrated birA gene does not require antibiotics to be maintained. AVB100 are compatible with IPTG-inducible vectors such as Avidity's pAC or pAN. AviTag vectors allow independent control over the expressed gene of interest and the BirA levels.

Avidity, LLC provides glycerol stocks (AVB100) or electro-competent cells (EVB100).

IMPORTANT NOTE:

Purchase of EVB100 includes a license for use only at site of purchase and may not be distributed to other sites.

License: Strain EVB100 or derivatives when purchased conveys a limited use license to the end user. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Avidity, L.L.C. Please review this license on the specification sheet below before purchasing this product.

  • What antibiotic do I have to use?

    The EVB100 strain itself is not carrying any antibiotic resistance. The plasmid that you are using for electroporation will carry the antibiotic resistance. For example Ampicillin for AviTag pAN and pAC vectors.