AVB100, an E. coli K12 strain [MC1061 araD139 delta (ara-leu)7696 delta(lac)l74 galU galK hsdR2(rK-
mK+) mcrB1 rpsL(StrR)], has a birA gene stably integrated into the chromosome. Overexpression of the
BirA protein is accomplished by induction with L-arabinose (0.2-0.4%). The stably integrated birA gene
does not require antibiotics to be maintained. AVB100 are compatible with IPTG-inducible vectors such
as Avidity's pAC or pAN. AviTag vectors allow independent control over the expressed gene of interest
and the BirA levels.
Avidity, LLC provides glycerol stocks (AVB100) or electro-competent cells (EVB100).
Purchase of AVB100 includes a license for use only at site of purchase and may not be distributed to
License: Strain AVB100 or derivatives when purchased conveys a limited use license to the end user.
This license prohibits the purchaser from selling, assigning or transferring this product to any third party
without the express written consent of Avidity, L.L.C. Please review this license on the specification
sheet below before purchasing this product.
Will AVB100 grow on LB-plates with antibiotics?
The untransformed cells will not grow if any antibiotics are present. These cells do not carry any antibiotic resistance, unless transformed with a plasmid (e.g. pAN vectors with Ampicillin resistance).
Why I’m not able to transform these cells with my plasmid of choice?
AVB100 are not made competent. Please use established protocols to make them competent or purchase electro-competent AVB100 cells, called EVB100.