Avidity brings the power of the biotin-avidin interaction to a whole new range of biological applications. Using Biotin AviTag™ Technology, it is now possible to biotinylate just about any protein easily and efficiently at a single, unique site. Avidity provides all the necessary materials to create a biotinylated protein that will launch your research and discoveries to new levels.

 
 
 
 
 
 
Protocols

Avidity provides protocols for guided use of products utilized in AviTagTM Technology. If further assistance is needed, please contact our technical support at 1-877-333-6063.

Induction of bacterial strains for in vivo biotinylation of AviTag™ proteins

General reaction conditions for biotin ligase


In vitro labeling purified AviTag™ proteins with biotin ligase

In vitro labeling AviTag™ proteins in BACTERIAL EXTRACTS

In vitro labeling AviTag™ proteins in MAMMALIAN EXTRACTS

In vitro labeling AviTag™ proteins on CELL SURFACES

In vitro labeling MHC MOLECULES with AviTag™

Guidelines for Anti C-terminus AviTag monoclonal antibody (AbCA)


Purifying C-terminus AviTag proteins with AbCA agarose


Determine the extent of biotinylation with BIS-300 (BRTA assay)