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 New Products
AvidExpressTM Cell-Free Translation System
AvidExpress™ cell-free translation system provides human HeLa S3 cell-derived translation factors, amino acids, chaperons and membranes for translation of cellular or in vitro synthesized mRNAs.
Features:
- Human cell lysate-based translation system
- The method is simple: just add supplied components and your RNA template.
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Just as with natural mRNAs, in vitro transcribed RNAs can be efficiently translated
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The translation products can be easily labeled with [35S]Methionine or biotinylated amino acids or use our AviTag sequence in your mRNA for detection of translated protein.
- Lysates can be used to study protein-protein, RNA-protein and DNA-protein interactions by UV cross-linking, electrophoresis mobility shift assay (EMSA) and immunoprecipitation assays.
- The system can be scaled-up easily.
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Biotinylated proteins can be synthesized for direct immobilization on solid surfaces coated with streptavidin or its molecular analogues
Biotin solution
Biotinylated Gaussia luciferase
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