Avidity brings the power of the biotin-avidin interaction to a whole new range of biological applications. Using Biotin AviTagTM Technology, it is now possible to biotinylate just about any protein easily and efficiently at a single, unique site. Biotin ligase, in the presence of ATP, specifically attaches biotin to the lysine residue in the Avitag peptide.

 
 
 
 
 
 
 
 
 
 
In vitro Biotinylation of Avitag with Enzyme

BIOTIN PROTIEN LIGASE
Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5' adenylate and transfers the biotin to biotin-accepting proteins. It also functions as a biotin operon repressor. This protein is encoded by the birA gene.

Other names for this enzyme include: biotin ligase; biotin operon repressor protein; birA; biotin holoenzyme synthetase; biotin-[acetyl-CoA carboxylase] synthetase.

Avidity sells purified biotin ligase
BirA 500 40ug Biotin ligase 1 mg/ml
Bulk BirA 300 ug Biotin ligase 3 mg/ml


Contact Avidity (877.333.6063) for quotes on larger orders of Biotin ligase
Specification sheet on enzyme

In Vitro Labeling Protocols when Avitag'd protein is:
Purified
In Bacterial extracts
In mammalian extracts
On cell surfaces
On MHC tetramers

Related products
BIS-300 positive and negative control protein kit
BIO-200 Biotin solution 1 mL 500 µM d-biotin solution (10 mM Bicine, pH 8.3)  
BIO-500 Biotin solution 10 mL 5 mM d-biotin solution (10 mM Bicine, pH 8.3)