Avidity has cloned and purified various proteins that have the AviTAG peptide which enables that protein to be biotinylated. The uses of such proteins are vast and only your imagination is the limit. 
 
 
 
 
 
 
 

New Products

Gaussia princeps Luciferase

Biotinylated Avitag™ fusion (C’terminus)

Gaussia princeps luciferase is a bioluminescent protein. It is ATP-independent, requiring only coelenterazine and O2 as substrates. This purified protein is fused with Avitag™ peptide which is fully biotinylated (MW 21,616 daltons). Gaussia princeps luciferase provides all the research possibilities of the biotin interaction with Streptavidin AND the applications of bioluminescence.

Gaussia luciferase from Gaussia princeps (a marine copepod) was genetically engineered to be produced in E.coli and to contain the Avitag peptide sequence.  Avitag peptide (GLNDIFEAQKIEWHE) can be efficiently biotinylated by the E.coli enzyme, biotin ligase .  The peptide can be recognized by Abc antibody.

Besides containing a biotinylated Avitag peptide, Gaussia luciferase has very unique properties as a bioluminescent protein.

  • Gaussia princeps luciferase is the smallest luciferase isolated to date. (MW 21,616 daltons with Avitag) 
  • Greater brightness compared to other luciferases
  • It has a spectral peak at 480 nm
  • It is sodium dependent
  • Similarly to Renilla luciferase, it is ATP independent and uses the substrate coelentrazine.
  • pH resistance (surviving a pH range of 3-11);
  • Good thermostability (up to 60°C and approx. 20% recovery following a 15 minute incubation at 99°C);
  • Activity even in the presence of non-ionic detergents (1-5% nonionic detergents (NP-40, Triton X-100, Triton X-114, CHAPSO);
  • Resistance to cholate, deoxycholate etc. and ability to recover activity after treatment with 7M guanidine chloride or 8M urea+NP-40

Specification Sheet

References:

Serganova I, Moroz E, Moroz M, Pillarsetty N and Blasberg R. (2006) Non-invasive molecular

imaging and reporter genes. Central European Journal of Biology. 1: pp. 88-123.
 

Tannous BA, Kim DE, Fernandez JL, Weissleder R and Breakefield XO. (2005) Codon-optimized

Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol. Ther. 11: pp.

435–443.
 

Verhaegen M and Christopoulos TK. (2002) Recombinant Gaussia luciferase. Overexpression,

purification and analytical application of a bioluminescent reporter for DNA hybridization. Anal. Chem.

74: pp. 4378–4385.