Avidity brings the power of the biotin-avidin interaction to a whole new range of biological applications. Using Biotin AviTag™ Technology, it is now possible to biotinylate just about any protein easily and efficiently at a single, unique site. Avidity provides all the necessary materials to create a biotinylated protein that will launch your research and discoveries to new levels.

 
 
 
 
 
 
In vitro labeling AviTag™ proteins on CELL SURFACES

Recently, the research group of Alice Ting at MIT has developed extensive protocols on the labeling of AviTag proteins on Cell Surfaces.
 
Howarth, M and Ting, A.Y.  (2008) Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin  Nature Protocols. 3(3) : 534-545. This can be retreived from the website:
 
The authors refer to the Avitag peptide as "the acceptor peptide or AP".  Avidity would like to remind commercial users of this protocol that the Avitag peptide is protected by U.S. Patent Nos. 5,723,584; 5,874,239; 5,932,433; 6,265,552.  Licenses for the use of Avitag peptide can be obtained through Avidity, LLC.  Further information
 
Avidity also sells biotin ligase and other products that may be useful in your research.