Biotin Protiein Ligase


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Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5'-adenylate and transfers the biotin to biotin-accepting proteins (such as Avitag peptide).  It also functions as a biotin operon repressor.  The protein is encoded by the birA gene. 
 
Please Note: We strongly recommend the use of the AviTag amino acid sequence over other Biotinylation of Peptides sequences. The BirA enzyme biotinylates the AviTag sequence at a reaction rate 2x the natural substrate (BCCP) and as much as an order of magnitude or more over the other peptide sequences in use. The AviTag requires smaller amounts of enzyme and shorter incubation times than other Biotinylation of Peptides sequences thus minimizing ancillary problems associated with proteases and protein instability.
SKU Description Price Order
BirA500
Concentration: 1 mg/ml. 
Activity: 5000 Units/µg. 
Quantity:  2 vials of 20 µg;  (200,000 Units)
 
Enzyme orders come with reaction buffers and
biotin solution for in vitro assays.
 
 		Price: $250.00
Quantity: