Avidity - BIP-300 positive control plasmid

BIP-300 positive control plasmid

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The β-galactosidase gene (8 amino acids deleted from N-terminus) was cloned into one of the Avidity N-terminus vectors (PAN5) creating a fusion protein of AviTag-β-gal. The plasmid can be transformed into bacterial strain background of interest by conventional means. The strain can then be induced in parallel with AviTag-protein of interest according to the following induction procedure. The following procedure is for AviTagged-protein in the Avidity strain AVB101. AVB101 contains the chloramphenicol resistant plasmid pACYC-184 that overexpresses the Biotin Ligase, BirA. If your strain is different, just disregard the use of chloramphenicol.

Product	SKU	Description	Price	Order
BIP-300 positive control plasmid	BIP300	Plasmid DNA containing Avitag fused to B-galactoside on the N-terminus. Quantity: 1 vial of 2mg purified DNA	Price: $150.00	Quantity:


AVB 99 Glycerol Stock	Biotin Protiein Ligase	BIO-500	CVB101 – Chemically Competent Cells of Efficient Biotinylation E. coli B Strain AVB101