AVB 99 Glycerol Stock


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Strain AVB99 is a K12 E. coli strain (genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1) containing pBirAcm, an engineered pACYC184 plasmid with an IPTG inducible birA gene to over-express the biotin ligase. The endA1 nuclease deficient strain is recommended for isolation of the pBirAcm plasmid that can be used to transform other E. coli strains to a biotin ligase over-expressing strains. The pBirAcm plasmid should be maintained and selected with chloramphenicol at 10 µg/ml concentration. This is a low copy number plasmid. Avidity, LLC provides glycerol stocks (AVB99)
 
SKU Description Price Order
AVB99
Catalog # AVB99 – Dnase deficient K-12 E. coli for making pBirAcm plasmid
 
Supplied as a 0.5 ml liquid culture in 30% glycerol.
Store at -80°C.
 
 
 		Price: $250.00
Quantity: