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AVB 99 Glycerol Stock
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Strain AVB99 is a K12
E. coli
strain (genotype:
rec
A1
end
A1
gyr
A96
thi
-1
hsd
R17
sup
E44
rel
A1) containing pBirAcm, an engineered pACYC184 plasmid with an IPTG inducible birA gene to over-express the biotin ligase. The
end
A1 nuclease deficient strain is recommended for isolation of the pBirAcm plasmid that can be used to transform other E. coli strains to a biotin ligase over-expressing strains. The pBirAcm plasmid should be maintained and selected with chloramphenicol at 10 µg/ml concentration. This is a low copy number plasmid. Avidity, LLC provides glycerol stocks (AVB99)
Product
SKU
Description
Price
Order
AVB99
AVB99
Catalog # AVB99 – Dnase deficient K-12 E. coli for making pBirAcm plasmid
Supplied as a 0.5 ml liquid culture in 30% glycerol.
Store at -80°C.
Price: $250.00
Quantity:
BIS-300 positive and negative control protein kit
AVB100 Glycerol Stock
Biotin Protiein Ligase
BIP-300 positive control plasmid