AVB101 is an E. coli B strain that will co-express biotin ligase and your gene of interest.

Strain AVB101, an E. coli B strain (hsdR, lon11, sulA1), contains pBirAcm , an IPTG inducible plasmid containing the BirA gene engineered into pACYC184. It is compatible with most cloning vectors and is maintained with chloramphenicol (10 µg/ml). This strain is recommended for protein expression because of its robust growth and the absence of the OmpT and Lon proteases. Avidity, LLC provides the strain in  three forms- glycerol stock (AVB101), chemically competent cells (CVB101), and electrocompetent cells (EVB101). Learn more about this product.


AVB 101 - Glycerol Stock
Strain AVB101 is an E. coli B strain (hsdR, lon11, sulA1) contains pBirAcm, an engineered pACYC184 plasmid with an IPTG inducible birA gene to over-express the biotin ligase.  This glycerol stock of bacteria are shipped frozen.  Learn more about this product.
 
 
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Price: $250.00
 
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CVB101 – Chemically Competent Cells of Efficient Biotinylation E. coli B Strain AVB101
AVB101 cells treated with CaCl2 to make them competent for the uptake of DNA.  Efficiency of transformation is >105 transformants per µg of supercoiled plasmid DNA.
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If the strain AVB101 has NOT been previously purchased, first-time purchase of CVB101 is $250 and subsequent purchases are at the price shown below.
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In Stock: Yes
Price: $175.00
 
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EVB101 – Electrocompetent Competent Cells of Efficient Biotinylation E. coli B Strain AVB101
AVB101 cells made competent for uptake of plasmid DNA by electroporation. Efficiency of transformation is >1010 transformants per µg of supercoiled plasmid DNA. Learn more about this product.
 
If the strain AVB101 has NOT been previously purchased, first-time purchase of CVB101 is $250 and subsequent purchases are at the price shown below.
 
 
 
 
 
Base SKU:EVB101
In Stock: Yes
Price: $175.00
 
Quantity: