Biotin Ligase

   BIO 200 (Avidity Biotechnologies)
BIO 200
Description:
Biotin solution
1 mL 500 µM d-biotin solution (10 mM Bicine, pH 8.3). Suitable for supplimenting in vitro biotin ligase reactions.
Base SKU: BIO200
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   BIO-500 (Avidity Biotechnologies)
BIO-500
Description:
Biotin solution
 
10 ml Biotin solution at higher concentration (5 mM) in 10 mM Bicine (pH 8.3).  Suitable for supplimenting induction growth media and large scale biotin ligase reactions.  Solution is filter sterilized. Store at 4oC.
Base SKU: BIO500
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   Biotin Protein Ligase (Bulk) (Avidity Biotechnologies)
Biotin Protein Ligase (Bulk)
Description:
Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5'-adenylate and transfers the biotin to biotin-accepting proteins (such as Avitag peptide).  It also functions as a biotin operon repressor.  The protein is encoded by the birA gene. 
 
Please Note: We strongly recommend the use of the AviTag amino acid sequence over other Biotinylation of Peptides sequences. The BirA enzyme biotinylates the AviTag sequence at a reaction rate 2x the natural substrate (BCCP) and as much as an order of magnitude or more over the other peptide sequences in use. The AviTag requires smaller amounts of enzyme and shorter incubation times than other Biotinylation of Peptides sequences thus minimizing ancillary problems associated with proteases and protein instability.
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   Biotin Protiein Ligase (Avidity Biotechnologies)
Biotin Protiein Ligase
Description:
Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5'-adenylate and transfers the biotin to biotin-accepting proteins (such as Avitag peptide).  It also functions as a biotin operon repressor.  The protein is encoded by the birA gene. 
 
Please Note: We strongly recommend the use of the AviTag amino acid sequence over other Biotinylation of Peptides sequences. The BirA enzyme biotinylates the AviTag sequence at a reaction rate 2x the natural substrate (BCCP) and as much as an order of magnitude or more over the other peptide sequences in use. The AviTag requires smaller amounts of enzyme and shorter incubation times than other Biotinylation of Peptides sequences thus minimizing ancillary problems associated with proteases and protein instability.
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   BIS-300 positive and negative control protein kit2 (Avidity Biotechnologies)
BIS-300 positive and negative control protein kit2
Description:

Kit contains fully biotinylated MBP-AviTag fusion protein and un-biotinylated MBP-AviTag fusion protein. Proteins can be used as controls in biotin ligase reactions, or gel analysis.  The fully biotinylated MBP-Avitag fusion protein can be used as a standard for determining the extent of biotinylation following the BRTA protocol.  Learn more about this product.
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